Effects of Ivabradine on IL—10 and IL—17 expression in myocardial cells of rabbit after myocardial infarction
綜合醫學
作者：戴偉
Abstract： Objective To investigate the effects of ivabradine （Iva） on IL-10 and IL-17 expression in myocardial cells of rabbit after myocardial infarction （MI） and its role in improving ventricular remodeling. Methods A total of 46 New Zealand white rabbits were used to build myocardial infarction model through ligating left anterior descending coronary artery. The successfully prepared 36 rabbis were randomly divided into 4 groups， 9 rabbits for each group： sham operation group （S group， thoracotomy without ligation）， myocardial infarction group （MI group）， bisoprolol treatment group （B group） and ivabradine treatment group （I group）. Drugs were given 12 hours post myocardial infarction induction， rabbits in B group and I group were given bisoprolol 1.25 mg/d and ivabradine 17 mg/kg/d respectively for 4 weeks； rabbits in S group and MI group were given same dose of saline at the same time for 4 weeks. After 4 weeks， body mass， left and ventricular mass were weighed， and IL-10 and IL-17 expression in myocardial cell was detected by immunohistochemistry method. Results Four weeks after operation， the relative ventricular mass of rabbits in MI group was increased compared with S group （P
Key Words： myocardial infarction； rabbit； interleukins； IL-10； IL-17.
After myocardial infarction （MI）， myocardial cells will produce many inflammatory cytokines， such as IL-17， IL-10 and so on 1. IL-17 can promote inflammatory response， and IL-10 can inhibit inflammatory response. The expression level of these two cytokines with opposite effect in myocardium may be associated with ventricular remodeling after MI. In this study， the effects of ivabradine and bisoprolol on IL-10 and IL-17 expression in myocardial cells of rabbits after myocardial infarction was observed， so as to explore the possible mechanism of both drugs in improving ventricular remodeling and asses the application value of ivabradine in improving acute myocardial infarction prognosis.
METHODS
1.1 Animals and grouping
A total of 46 New Zealand white rabbits （male or female）， with body mass 2 - 3kg， were provided by Qingdao Drug Administration Station. Rabbit myocardial infarction models were prepared according to the method of Liu Bin etc. 2. Electrocardiogram showed arched elevation of precordial ST segment， which did not fall back within 30 minutes， suggesting myocardial infarction models were successfully prepared， and then conducted sternal closure layer by layer. After operation， the rabbits were given penicillin sodium 400，000 U/d through conventional intramuscular injection once every three days to prevent infection. During the operation， 10 rabbits were died of anesthesia accident and pneumothorax， and survived rabbits were randomly divided into 4 groups： myocardial infarction group （successful modeling； MI group）， bisoprolol treatment group （treatment with bisoprolol after successful modeling； B group）， ivabradine treatment group （treatment with ivabradine after successful modeling； Iva group） and sham group （S group， only thoracotomy without ligation）， 9 rabbits for each group. Rabbits in B group were given 1.25mg/d bisoprolol， ivabradine （Wuhan Yinhe Chemical Co.， Ltd.， batch number 20110201） was given with the dosage of 17mg/kg/d. Rabbits in S and MI group were given same dose of saline at the same time.