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Investigation on formation mechanism of secologanic acid sulfonates in
sulfur-fumigated buds of Lonicera japonica
GUO Ai-li 1，2，3， GAO Hui-min 2，3*， CHEN Liang-mian 2，3， ZHANG Qi-wei 2，3， WANG Zhi-min 2，3*
（1.School of Traditional Chinese Medicine， Capital Medical University， Beijing 100069， China；
2.Institute of Chinese Materia Medica， China Academy of Chinese Medical Sciences， Beijing 100700， China；
3.National Engineering Laboratory of Quality Control Technology of Chinese Herbal Medicines， Beijing 100700， China）
[Abstract] To investigate formation mechanism of secologanic acid sulfonates in sulfur-fumigated buds of Lonicera japonica， secologanic acid was enriched and purified from the sun-dried buds of L. japonica by various column chromatography on macroporus resin HPD-100， silica gel and ODS. The stimulation experiments of sulfur-fumigation process were carried out using secologanic acid reacted with SO2 in the aqueous solution. The reaction mechanism could be involved in the esterification or addition reaction. The present investigation provides substantial evidences for interpreting formation pathway of secologanic acid sulfonates in sulfur-fumigated buds of L. japonica.
[Key words] sulfur-fumigation； Lonicera japonica； secologanic acid
doi：10.4268/cjcmm20140919