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Simultaneous determination of ten active ginsenosides in
steamed notoginseng by UPLC
CHEN Bin1*， CAI Tao2， JIA Xiao-bin1
（1.Key Laboratory of Chinese Medicine Delivery System of State Administration of Traditional Chinese Medicine，
Jiangsu Provincial Academy of Chinese Medicine， Nanjing 210028， China；
2.Jiangsu Huaian Liberation Army 82th Hospital， Pharmaceutical Preparation Section， Huaian 223001， China）
[Abstract] A quantitative method using ultrahigh-performance liquid chromatography was established to simultaneously determine ten ginsenoside active ingredients including ginsenoside Rg6， F4，Rk3， Rh4， 20（S）-Rg3， 20（R）-Rg3， Rk1， Rg5， 20（S）-Rh2 and 20（R）-Rh2 in steamed notoginseng. The ten ginsenosides of steamed notoginseng with different head numbers， parts， and steaming time were determined by this method. An Acquity BEH C18 chromatographic column （2.1 mm×100 mm，1.7 μm） was used to perform the determination， which was maintained at 35 ℃ throughout the analysis. Mobile phase was composed of water and acetonitrile with flow rate at 0.3 mL·min-1 under gradient elution， and detection wavelength was set to 203 nm for monitoring the separation. The results demonstrate ginsenoside Rg6， F4， Rk3， Rh4， 20（S）-Rg3， 20（R）-Rg3， Rk1， Rg5， 20（S）-Rh2 and 20（R）-Rh2 have shown good linearity （R2≥0.999 8） within 0.46-115， 2.06-515， 1.632-408， 3.216-804， 1.392-348， 1.4-350， 0.496-248， 3.012-1 506， 0.82-205 and 0.832-208 mg·L-1， and their average recoveries were 97.00%， 97.96%， 98.86%， 95.27%， 98.67%， 98.02%， 95.53%， 96.63%， 99.57% and 103.6%， respectively. The proposed approach was quick and accurate and portrayed excellent repeatability and determination efficiency. The quality of steamed notoginseng was effectively controlled， which served as a foundation for establishing a normalized processing technique and quality standard for ensuring the reliability and consistency of its clinical efficacy.
[Key words] steamed notoginseng； ginsenoside； ultrahigh-performance liquid chromatography； simultaneous multicomponent determination； quality control
doi：10.4268/cjcmm20140914