委陵菜積雪草酸對大鼠酒精性肝損傷的保護作用
藥理
作者：趙趕 呂淑娟 韋剛 韋錦斌 林興 黃權芳
[摘要]研究委陵菜積雪草酸（asiatic acid， AA）對大鼠酒精性肝損傷的保護作用及其機製。將Wistar雄性大鼠隨機分成正常對照組，AA對照組（8 mg·kg-1AA），模型組（5.0～9.0 g·kg-1乙醇），及AA高、中、低劑量治療組（乙醇+8，4，2 mg·kg-1AA）。各組灌胃給予相應的藥物，每天1次，連續24周。末次給藥1.5 h 後，取血，處死大鼠，采集肝組織。檢測大鼠血清AST，ALT，檢測肝髒髓過氧化物酶（MPO），腫瘤壞死因子（TNF-α），白細胞介素1（IL-1β），超氧化物歧化酶（SOD），GSH-Px，GSH-Rd，丙二醛（MDA）的水平；采用Western blot法檢測肝組織中NF-κB，Toll樣受體4（TLR4），CD14，髓樣分化因子88（MyD88）和TIR結構域銜接蛋白-β（TRIF），蛋白表達情況；同時做組織學檢查，觀察肝組織的病理變化。結果顯示與模型組相比較，AA治療後，大鼠血清ALT和AST水平，MDA含量明顯降低，SOD，GSH-Px，GSH-Rd，MPO活性明顯升高。此外，AA能抑製TNF-α，IL-1β的表達；同時還可抑製TLR4，CD14，MyD88以及核因子NF-κB的水平。組織學檢查顯示，大鼠肝組織損傷程度均有所減輕。綜合以上結果可知委陵菜積雪草酸對乙醇所致的大鼠肝損傷具有一定的保護作用，其機製可能與抑製NF-κB活化減輕炎症反應有關。
[關鍵詞]委陵菜；積雪草酸；酒精；肝損傷
Protective effect of asiatic acid from Potentilla chinensis on
alcohol hepatic injury in rats
ZHAO Gan1， LV Shu-juan2， WEI Gang4， WEI Jin-bin2， LIN Xing2， HUANG Quan-fang3*
（1.Guangxi Zhuang Autonomous Region Maternal and Child Health Hospital， Nanning 530003， China；
2. Guangxi Medical University， Nanning 530021， China；
3. The First Affiliated Hospital of Guangxi Traditional Chinese Medical University， Nanning 530023， China；
4. Guangxi Research Center of Analysis and Testing， Nanning 530022， China）
[Abstract]To study the protective effect and the mechanism of asiatic acid （AA） from Potentilla chinensis on alcohol hepatic injury in rats. Male Wistar rats were randomly divided into six groups： the normal control group， the AA control group （8 mg·kg-1 AA）， the model group （5.0-9.0 g·kg-1 alcohol） and high， medium and low-dose AA-treated groups （alcohol + 8， 4， 2 mg·kg-1 AA）. Each group was orally administered with the corresponding drugs once a day for 24 weeks. Approximately 1.5 hours after the final administration， all rats were killed， and their blood samples and hepatic tissues were collected. The AST and ALT in rat serum and the contents of MPO， TNF-α， IL-1β， SOD， GSH-Px， GSH-Rd and MDA in hepatic tissues were detected. The expressions of NF-κB， TLR4， CD14， MyD88， TRIF and protein expression in hepatic tissues were measured by western blot. The pathological changes in liver tissues were observed by histological examination. The results showed that compared with the model group， the AA-treated groups showed significant decreases in serum ALT， AST and MDA and increases in the activities of SOD， GSH-Px， GSH-Rd and MPO. Moreover， AA markedly inhibited the expressions of TNF-α， IL-1β， TLR4， CD14， MyD88 and NF-κB. The histological examination showed alleviated hepatic issue ijury to varying degrees. In short， asiatic acid （AA） from P. chinensis could protect alcohol-induced hepatic injury in rats. Its mechanism may be related to the inhibition of NF-κB inactivation and the reduction of inflammatory response.