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Isolation and mass spectrometric analysis of antioxidant peptides from
enzymatic hydrolysates of roasted pills of Asini Corii Colla
LI Xiao，chen YAN Li，hua2，3*， WANG Zhi，min2，3*， ZHANG Qi，wei2，3， GAO Jian，ping4，
CHEN Liang，mian2，3， WANG Jin，yu TONG Yan ZHANG Gui，feng4
（1. School of Traditional Chinese Medicine， Capital Medical University， Beijing ， China；
2. Institute of Chinese Materia Medica， China Academy of Chinese Medical Sciences， Beijing ， China；
3.National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines， Beijing ， China；
4. Institute of Process Engineering， Chinese Academy of Sciences， Beijing ， China）
[Abstract] A fraction named GFC，1 with high antioxidant activities in vitro was isolated from the enzymatic hydrolysates of roasted pills of Asini Corii Colla， and the peptides in this fraction were identified. The enzymatic hydrolysates were isolated and purified with anion exchange chromatography and Sephadex G，25 filtration chromatography successively. GFC，1， a fraction isolated from the hydrolysates， exhibited the highest DPPH and ABTS scavenging capacity （DPPH 47.95%at 2.0 g·L-1 and ABTS 97.20%at 0.40 g·L-1）. Nine peptides from GFC，1 were identified by LC，ESI，MS/MS coupled with TurboSEQUEST search software and Swiss，Prot data base， and a high repetition core sequence GPAGPP*GPP* was also found.
[Key words] Asini Corii Colla； antioxidant peptides； LC，ESI，MS/MS； amino acid sequence
doi：10.4268/cjcmm