[摘要] 目的 探討黃芪對大鼠慢性胰腺炎的治療作用。方法 采用光度酶聯免疫分析法(ELISA)及比色法檢測胰腺正常組、對照組、幹預組中的層粘連蛋白(LN)的含量及超氧化物歧化酶(SOD)的活性進行比較分析，同時對胰腺組織進行HE染色，觀察病理形態學改變。結果 胰腺纖維化組血清中LN的含量明顯增高，SOD的活性降低，幹預組中LN含量較對照組降低，SOD活性較對照組升高。結論 黃芪注射液有改善大鼠胰腺纖維化的作用。
[關鍵詞] 黃芪注射液； 胰腺纖維化； 層粘連蛋白； 超氧化物歧化酶
[中圖分類號] R255.5 [文獻標識碼] A [文章編號] 1673-9701(2010)03-03-03
Therapeutic Effect of Astragalus Injection on Chronic Pancreatitis
GUO Qiang ZHANG Bingtai LI Maolan REN Hui
Department of General Surgery，the Second Hospital of Shanxi Medical University，Taiyuan 030001，China
[Abstract] ObjectiveTo investigate the therapeutic effect of Astragalus injection on rat chronic pancreatitis. MethodsThe laminin(LN)level and the superoxide dismutase(SOD) activity were detected in normal pancreas group(pancreas fibrosis group)，control group and intervention group by using enzyme immunoassay(ELISA) and colorimetric method，and the pancreatic tissue was stained by using HE and its pathological change was observed. ResultsThe serum LN level was significantly higher and the SOD activity was decreased in the pancreas fibrosis group. The serum LN level was lower and the SOD activity was higher in the intervention group compared with the control group. ConclusionAstragalus injection has the role in the improvement of rat pancreatic fibrosis.
[Key words]Astragalus injection； Pancreatic fibrosis；Laminin；Superoxide dismutase
慢性胰腺炎是由於膽道疾病或酒精中毒等因素導致的胰腺實質進行性損害和纖維化，常伴鈣化、假性囊腫及胰島細胞減少或萎縮。主要表現為腹痛、消瘦、營養不良、腹瀉或脂肪痢，後期可出現腹部包塊、黃疸和糖尿病等。其病理生理過程複雜，以胰腺內成纖維細胞和細胞外基質(ECM)在胰腺組織內過多沉積為其特征[1]。傳統的胰腺纖維化指標是LN的含量升高及SOD活性降低。我們采用光度酶聯免疫分析法(ELISA)檢測胰腺正常組、對照組、黃芪注射液小劑量組、中劑量組、大劑量組中的LN的含量及SOD的活性進行比較分析，以初步探討其對胰腺纖維化的治療作用。
1 材料與方法
1.1 實驗動物
選用雄性Spraque-Dawley大鼠50隻，清潔級，體重200～250g，由上海斯萊克實驗動物有限公司提供，飼養於山西醫科大學動物實驗中心。