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Effect of triptolide on iNOS and SP expressions in spinal dorsal horn and
dorsal root ganglion of rats with adjuvant arthritis
CHEN Wei， ZHANG Xu-dong， LU Zhuo-hui， WEI Deng-ming*
（Department of Pathology， School of Medicine， Ningbo University， Ningbo 315211， China）
[Abstract] Objective： To observe the analgesic effect of triptolide （TP） of high， middle and low doses on rats with adjuvant arthritis （AA）， and the expressions of inducible nitric oxide synthase （iNOS） and substance P（SP） in spinal dorsal horn and dorsal root ganglion（DRG） of corresponding sections， in order to discuss the possible mechanism for the analgesic effect of TP on rats with adjuvant arthritis. Method： Fifty SD rats were selected and randomly divided into the normal group（group A）， the model group（group B）， and TP low（group C）， middle（group D）， high（group E） dose groups. Except for the group A， all of the remaining groups were injected with 0.1 mL of freund′s complete adjuvant through their right rear toes to establish the model. At 14 d after the model establishment， rats in C， D and E groups were intraperitoneally injected with different doses of TP （0.1 mg·kg-1 for the group C， 0.2 mg·kg-1 for the group D， 0.4 mg·kg-1 for the group E） once a day for 9 days. Then the 50% mechanical withdraw threshold （MWT） was determined. And the expressions of iNOS and SP in lumbar5 （L5） spinal dorsal horn and DRG were detected with the immunohistochemical method. Result： The 50% MWT of rats in the group B was significantly lower than that of the group A（P
[Key words] adjuvant arthritis； triptolide； analgesia； inducible nitric oxide synthase； substance P
doi：10.4268/cjcmm20140926