[13] Zhang H， Li Q， Li Z， et al. The protection of Bcl-2 overexpression on rat cortical neuronal injury caused by analogous ischemia/reperfusion in vitro[J]. Neurosci Res， 2008， 62（2）：140.
[14] Chen A， Lin Z， Lan L， et al. Electroacupuncture at the Quchi and Zusanli acupoints exerts neuroprotective role in cerebral ischemia-reperfusion injured rats via activation of the PI3K/Akt pathway[J]. Int J Mol Med， 2012， 30 （4）：791.
[15] Peng B， Guo Q L， He Z J， et al. Remote ischemic postconditioning protects the brain from global cerebral ischemia/reperfusion injury by up-regulating endothelial nitric oxide synthase through the PI3K/Akt pathway[J]. Brain Res， 2012， 1445：92.
[16] Gao X W， Zhang H F， Steinberg G， et al. The Akt pathway is involved in rapid ischemic tolerance in focal ischemia in rats[J]. Transl Stroke Res， 2010， 1（3）：202.
Effect of Schisandra chinensis lignans on neuronal apoptosis and
p-AKT expression of rats in cerebral ischemia injury model
JIANG En-ping 1， 2， WANG Shuai-qun1，4， WANG Zhuo4， YU Chun-rong5， CHEN Jian-guang1*， YU Chun-yan3*
（1.College of Pharmacy， Beihua University， Jilin 132001， China； 2. Guangdong Medical College， Dongguan 523808， China；
3. Basic Medical College， Beihua University， Jilin 132001， China； 4.Jilin Central General Hospital， Jilin 132001， China；
5. JOINN Laboratories， Beijing 100176， China）
[Abstract] Objective： To observe the effect of Schisandra chinensis lignans（SCL） on neuronal apoptosis and PI3K/AKT signaling pathway of rats in the cerebral ischemia injury model， and study its possible mechanism. Method： Rats were orally administered SCL high， middle and low dose groups （100， 50， 25 mg·kg-1） for 14 days. The cerebral ischemia injury model was established by using the suture-occluded method to rate the neurological functions. The cerebral infarction area was observed by TTC staining. The pathological changes in brain tissues were determined by HE staining. Bcl-2 and Bax expressions were detected by immunohistochemical assay. The protein expressions of p-AKT and AKT were assayed by Western blotting. Result： Compared with the model group， SCL high， middle and low dose groups showed reduction in the cerebral infarction area to varying degrees， improve the pathological changes in brain tissues， promote the expression of apoptin Bcl-2 and p-AKT， and inhibit the expression of apoptin Bax. Conclusion： SCL shows a protective effect on rats with cerebral ischemia injury. Its mechanism may be related to the increase in p-AKT ability and anti-ischemic brain injury capacity and the inhibition of nerve cells.
[Key words] Schisandra chinensis； cerebral ischemia； phosphatidylinositol 3-kinase； apoptosis
doi：10.4268/cjcmm20140927