[4] 湯喜蘭，劉建勳，李磊，等、廣棗模擬總有機酸對心肌缺血再灌注損傷的保護作用[J]、中國實驗方劑學雜誌，2013，19 （4）：168、
[5] Li P， Fu J H， Wang J K， et al、 Extract of Paris polyphylla Simth protects cardiomyocytes from anoxia-reoxia injury through inhibition of calcium overload[J]、 Chin J Integr Med， 2011， 17 （4）： 284、
[6] 李澎，任鈞國，段昌令，等、 4種延胡索成分對乳鼠心肌細胞缺氧和過氧化損傷的影響[J]、中國中藥雜誌，2010，35（1）：85、
[7] 湯喜蘭，劉建勳，李澎，等、山柰酚和槲皮素對乳鼠心肌缺氧複氧及過氧化損傷的保護作用[J]、中藥藥理與臨床，2012，28 （1）：57、
[8] 黃黎，任娜，葉文華、蓍草有機酸的藥理作用[J]、中藥通報，1985，10（11）：38、
[9] 叢紅群，嶽旺，楊誌宏，等、琥珀酸在海馬CA1區對突觸前GABA釋放的影響[J]、神經解剖學雜誌，2009，25（1）：6、
[10] Mullonkal C J， Toledo-Pereyra L H、 Akt in ischemia and reperfusion[J]、 J Invest Surg， 2007， 20 （3）： 195、
Protective effect of succinic acid on primary cardiomyocyte
hypoxia/reoxygenation injury
TANG Xi-lan1， 2， LIU Jian-xun1*， LI Peng1， DONG Wei1，2， LI Lei1， ZHENG Yong-qiu1， HOU Jin-cai 1
（1、 Experimental Research Center， Xiyuan Hospital， China Academy of Chinese Medical Sciences， Beijing ， China；
2、 Key Laboratory of Modern Preparation of Traditional Chinese Medicine， Jiangxi University of
Traditional Chinese Medicine， Nanchang ， China）
[Abstract] To establish cardiomyocyte hypoxia/reoxygenation injury model by culturing primary cardiomyocytes from suckling SD rats， in order to study the effect of succinic acid on LDH leakage rate cardiomyocyte ischemia/reperfusion injury、 Furthermore， flow cytometry and western blot were conducted to detect the effect of succinic acid on cardiomyocyte apoptosis， cleaved caspase-3 and p-Akt， and discuss the protective effect of succinic acid on primary cardiomyocyte hypoxia/reoxygenation injury of primary cardiomyocytes from neonatal SD rats、 According to the findings of the study， succinic acid at the concentrations ranging between 31、25 mg·L-1 and 500 mg·L-1 had no significant effect on primary cardiomyocyte activity， and succinic acid at the concentrations of 400， 200， 100， 50 mg·L-1 could notably reduce cardiomyocyte ischemia/reperfusion LDH leakage rate （P-1 and 200 mg·L-1 could significantly reduce the percentage of cardiomyocyte apoptosis （P-1 could remarkably increase the protein expression of cardiomyocyte Akt （P-1 had no obvious effect on the protein expression of cardiomyocyte Akt、 Therefore， this study demonstrated that succinic acid could inhibit necrosis and apoptosis caused by cardiomyocyte hypoxia/reoxygenation by activating Akt phosphorylation、
[Key words] succinic acid； cardiomyocyte； hypoxia/reoxygenation； necrosis； apoptosis
doi：10.4268/cjcmm
[責任編輯張寧寧]