2、2、4 Nestin免疫組化（DAB法）檢測采用2、2、3項中大腦石蠟切片經二甲苯脫蠟至水，常規免疫組化法進行Nestin染色，結果在顯微鏡下觀察。每隻小鼠取3張連續切片，每張切片在400倍光鏡下選取上、下、左、右、中5個視野，在拍攝條件相同的條件下拍照，並利用Image pro plus 6、0圖像處理係統分析視野陽性表達，平均吸光度=累積吸光度（IOD）/總麵積（Area），取9個視野平均值。
2、3 數據統計數據以±s表示，采用SPSS 12、0統計軟件處理，組間比較采用方差齊性檢驗，後利用單因素方差分析，P1， CHEN Xiao-yu1，2*， ZHUANG Shu-juan1， LI Ke-qin1
（1、Minzu University of China， State Nationalities Affairs Commission and Department of Educational Key Lab of
Minority Traditional Medicine， Beijing ， China； 2、 Tianjin Port Hospital， Tianjin ， China）
[Abstract] The neural stem cells （NSCs）， play a crucial role in stroke treatment， which can be regulated by a few of traditional Chinese medicines、 In this study， the effect of the Mongolian medicine Baimai powder effective compounds group （BMECG） on the proliferation of NSCs has been investigated、 The cultured NSCs which were isolated from newborn rat cerebral cortical in vitro were exposed to oxygen glucose deprivation/reoxgenation（OGD/R）、 The CFSE immunofluorescence staining was employed to identify the proliferation of NSCs by flow cytometry、 Furthermore， the bilateral carotid artery occlusion （BCAO） was established on Kunming mice， and all groups were ig for 7 d respectively、 The neurobehavioral changes was studied with rota-rod treadmill test， after that， the brain of mice were detected by immunohistochemistry with labeling of Nestin and pathological observation at 7 days after BCAO、 It was found that， proliferation of NSCs was increased by BMECG in in vitro and in vivo、 And BMECG significantly improved the time of staying in the rota-rod， it can promote the foundction of in cerebral cortex、 It is concluded that these results further support the hypothesis that neuroprotective effect of BMECG may relate to the ability of stimulating self-renew of NSCs， which can be provided a new insight and strategy of anti-neuropathy of stroke.
[Key words] Baimai powder； effective compounds group； neural stem cells； stroke； CFDA-SE
doi：10.4268/cjcmm[責任編輯張寧寧]