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Study on preparation process and formulation
optimization of herpetin liposomes
ZHANG Xin TAN Rui GU Jian HE Li-li FAN Li-na NAN Xing-mei1
（1.Ethinic Medicine Research Institute， Southwest University for Nationalities， Chengdu 610041， China；
2.Life Science and Engineering College， Southwest Jiaotong University， Chengdu 610031， China）
[Abstract] Herpetin （HPT） is an active monomer constituent isolated from lignanoid in seeds of Herpetospermum caudigerum. HPT shows inhibitory effects in hepatic injury and HBV-DNA and the replication. In the study， we successfully prepare herpetin liposomes by film dispersion method for the first time. The prescription process was optimized， with the entrapment efficiency as the index. According to the optimized prescription， the mass ratio of HPT： phospholipids： cholesterol was 2.44∶78.05∶19.51， the hydration and de-molding process was performed with 0.5% F68 solution at 50 ℃， and the water-bath ultrasonic time was 20 min. The HPT liposomes prepared by this method showed an average entrapment efficiency of （94.50±2.15）% and a particle size of （119.2±10.7） nm， which was consistent with the trial expectations and will lay a solid foundation for the hepatic targeting delivery system in future.
[Key words] herpetin； liposome； preparation process； prescription optimization
doi：10.4268/cjcmm20140610