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Difference in effect between asthma-based mouse
model and Stemona tuberosa extracts
CHEN Xiao-xia1， 3， ZHANG Xiao-dan1， LI Hong-yan1， JIA Tian-zhu1， 2*， YANG Jing-xian1
（1. Liaoning University of Traditional Chinese Medicine， Dalian ， China；
2. Key Laboratory of Traditional Chinese Medicine Processing Technology and Principle under State
Administration of Traditional Chinese Medicine， Dalian ， China；
3. Liaoning Research Center for Traditional Chinese Medicine Processing Technology， Dalian ， China）
[Abstract] In this study， OVA-induced asthma mice was taken as the model， and orally administered with different concentration of ethanol extracts of crude and processed Stemona tuberosa， in order to determine the cytokine level released from Th1 and Th2 in splenocytes. RT-PCR was carried out to determine the genetic expression of T-bet/GATA-3 in lung， and compare the differentiation between ethanol extracts of crude and processed S. tuberosa in therapeutic effect on asthma in mice. According to the results， compared with the crude samples， processed samples significantly increased the levels of inflammatory factor INF-γ（P
[Key words] crude and processed Stemona tuberosa； asthma； cytokine； GATA-3； T-Bet
doi：10.4268/cjcmm