4結論
中藥成分較為複雜，理化性質差異較大且結構類似的化合物較多，因此譜峰重疊現象較常見。通過係統的方法學驗證，表明本試驗所建立二維色譜分析方法，簡便快速，自動化程度高，且可同時進行桂枝茯苓膠囊中4種成分的含量測定。利用一維和二維色譜柱分離機製的差異，提高了係統的分離能力，消除了基質成分的幹擾，測定結果更加準確，對提高分析效率，控製藥物質量具有一定意義。
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[Key words] two-dimensional liquid chromatography； double gradient liquid chromatography； Guizhi Fuling capsule； amygdaloside； cinnamic acid
doi：10.4268/cjcmm