[9] Zhao L，Lou Z Y，Zhu Z Y，et al.Characterization of constituents in Stellera chamaejasme L. by rapid-resolution liquid chromatography-diode array detection and electrospray ionization time-of-flight mass spectrometry [J].Biomed Chromatogr，2008，22：64.
[10] Zhang H，Gong C G，Lv L，et al.Rapid separation and identification of furocoumarins in Angelica dahurica by high-performance liquid chromatography with diode-array detection，time-of-flight mass spectrometry and quadrupole ion trap mass spectrometry[J].Rapid Commun Mass Spectrom，2009，23：2167.
Rapid identification of chemical constituents in
Salvia chinensis by HPLC-TOF-MS
ZHOU Yan-ni， ZHAO Liang， ZHENG Lei， LV Lei*
（Department of Pharmacy， Eastern Hepatobiliary Surgery Hospital， Shanghai ， China）
[Abstract] It′s established a high-performance liquid chromatography-time of flight mass spectrometry（HPLC-TOF-MS）method to analyze chemical constituents in Salvia chinensis. The separation was performed on a SHISEIDO MG C18 reverse phase column（3.0 mm×100 mm，3 μm）.The mobile phase consisted of acetonitrile（A） and water （containing 0.1% formic acid，B） was used as gradient elute.The gradient of a phase，10%-90%（0-33 min），90%（33-40 min）. The flow rate was 0.6 mL·min-1. Post-column split ratio was 2∶1. Temperature of column was 25 ℃. Time-of-flight mass spectrometer（TOF-MS） and electrospray ion source（ESI） was applied for qualitative analysis under positive ion mode，and mass scan range was m/z 100-1 000. As a result，28 of the major chemical constituents of S.chinensis were identified by HPLC-TOF-MS. In this study，a rapid and efficient method for studying the chemical constituents in S.chinensis by HPLC-TOF-MS was established，which paves a way for the quality control and further studies of the herb in vivo.
[Key words] Salvia chinensis； chemical constituents； identification； TOF-MS
doi：10.4268/cjcmm